| 质粒名称: | pLenti CMV/TO p16 Hygro (w184-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22263 |
| 存储实验室: | Eric Campeau |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8722 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | cyclin-dependent kinase inhibitor 2A,p16 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 446 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bam HI (未破坏) |
| 3' 克隆位点: | Xba I (未破坏) |
| 5' 测序引物 | CMVforw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV/TO p16 Hygro (w184-1) was a gift from Eric Campeau (Addgene plasmid # 22263 ; http://n2t.net/addgene:22263 ; RRID:Addgene_22263) |
买家导航
pLenti CMV/TO p16 Hygro (w184-1)载体质粒基本信息
pLenti CMV/TO p16 Hygro (w184-1)质粒图谱载体图谱和pLenti CMV/TO p16 Hygro (w184-1)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pLenti CMV/TO p16 Hygro (w184-1)质粒载体简介
pLenti CMV/TO p16 Hygro (w184-1)质粒序列载体序列
LOCUS pLenti CMV/TO p16 Hygro (w184-1) 9168 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLenti CMV/TO p16 Hygro (w184-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9168)
TITLE shMDC1 plasmids
REFERENCE 2 (bases 1 to 9168)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9168
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(350..369)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1652..1671
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1905..1922
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2039..2060
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2075..2105
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2113..2129
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2118..2140
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2137..2153
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2137..2153
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2172..2192
/label=T3
/note="T3 promoter, forward primer"
promoter 2174..2192
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
LTR 2448..2628
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2672..2797
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3290..3523
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3707..3751
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4012..4129
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4152..4455
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4456..4659
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4610..4630
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 4661..4679
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4682..4700
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4741..4765
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(5293..5317)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 5348..5936
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5401..5421)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5819..5830)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 5992..6491
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6027..6050)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6424..6443
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
LTR 7909..8142
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 8214..8335
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8251..8270)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8305..8324
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8354..8489
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8416..8435
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8521..8540)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8522..8540)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8550..8567)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8550..8566)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8559..8581)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8711..9166
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8798..8817)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9008..9029
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
4681 ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct gcagatatca
4741 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccagcat
4801 ggagccttcg gctgactggc tggccacggc cgcggcccgg ggtcgggtag aggaggtgcg
4861 ggcgctgctg gaggcggggg cgctgcccaa cgcaccgaat agttacggtc ggaggccgat
4921 ccaggtcatg atgatgggca gcgcccgagt ggcggagctg ctgctgctcc acggcgcgga
4981 gcccaactgc gccgaccccg ccactctcac ccgacccgtg cacgacgctg cccgggaggg
5041 cttcctggac acgctggtgg tgctgcaccg ggccggggcg cggctggacg tgcgcgatgc
5101 ctggggccgt ctgcccgtgg acctggctga ggagctgggc catcgcgatg tcgcacggta
5161 cctgcgcgcg gctgcggggg gcaccagagg cagtaaccat gcccgcatag atgccgcgga
5221 aggtccctca gacatccccg attgaaagga attctgcaga tatccatcac actggccgct
5281 cgagatatct agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
5341 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
5401 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
5461 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
5521 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
5581 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
5641 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
5701 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
5761 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
5821 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
5881 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
5941 aattctgcag atatccggac tagtgatctc tcgaggttaa cgaattctac cgggtagggg
6001 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
6061 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
6121 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
6181 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
6241 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
6301 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
6361 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
6421 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
6481 cgggcctttc gacctgcatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt
6541 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
6601 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
6661 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
6721 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
6781 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
6841 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
6901 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
6961 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
7021 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
7081 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
7141 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
7201 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
7261 gaggcatccg gagcttgcag gatcgccgcg gctccggggc gtatatgctc cgcattggtc
7321 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
7381 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
7441 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
7501 gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg aacaagagct
7561 gatttcgaga acgcctcagc cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa
7621 cggccttacg cttggtggca cagttctcgt ccacagttcg ctaagctcgc tcggctgggt
7681 cgcgggaggg ccggtcgcag tgattcaggc ccttctggat tgtgttggtc cccagggcac
7741 gattgtcatg cccacgcact cgggtgatct gactgatccc gcagattgga gatcgccgcc
7801 cgtgcctgcc gattgggtgc agatccgtcg agggcccggt acctttaaga ccaatgactt
7861 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
7921 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
7981 agatctgagc ctgggagctc tctggctgcc tagggaaccc actgcttaag cctcaataaa
8041 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
8101 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
8161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
8221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
8281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
8341 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
8401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
8461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg
8521 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
8581 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
8641 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
8701 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
8761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
8821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
8881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
8941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
9001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
9061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
9121 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcc
//
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pLenti CMV/TO p16 Hygro (w184-1)质粒载体应用举例
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